From the beginning of February the supermarket giant will charge customers £2 for every click-and-collect order on non-food products worth less than £30.
Tesco explained the charge was designed to ensure its click-and-collect service "remains sustainable for the future".
John Lewis led the way in beginning to charge for click and collect in July by introducing a £2 charge on click and collect orders under £30.
Andy Street, managing director at John Lewis, explained the move was in order to ensure the service was sustainable by funding the logistical operation behind click-and-collect.
Retailers are battling to offer the most convenient fulfilment options in order to win customers’ spend.
Amazon is setting the bar high with constant fulfilment innovations including one-hour delivery for members of its Amazon Prime service.
Argos is fighting to keep up with same-day deliveries for £3.95 and commentators believe the strength of Argos’ fulfilment network is one of the key incentives for Sainsbury’s making an acquisition bid for Argos-owner Home Retail.
Tesco rival Asda offers click-and-collect free of charge, while Sainsbury’s offers it for free if customers spend £20.