Who We Be will take place at Alexander Palace in north London on 30 November and will also feature Giggs, J Hus and Stefflon Don.
The music event is an extension of a playlist on Spotify with the same name and more than 140,000 followers. The company is working with Amplify to put on the event and develop the Who We Be platform.
Earlier this week Apple announced the closure of its UK festival after ten years. The event – initially called the iTunes Festival and latterly known as Apple Music Festival – had featured acts including Elton John, Adele, Coldplay and Ed Sheeran.
Tickets for Who We Be are available to buy on Monday 11 November and cost £35.
Austin Daboh, senior editor at Spotify, said: "Spotify has worked tirelessly to support British talent over the last year, and we’re proud to have played a key role in the success of various urban genres such as grime and rap.
"Who We Be Live at Alexandra Palace is Spotify’s first ever concert in the UK and will bring together some of the biggest and most exciting artists under one roof for an unforgettable night."